Study the characters of poly-β-hydroxybutyrate isolated from local sample in Babylon

Abstract

       The most of Bacillus species was able to produce poly-β-hydroxybutyrate isolated from soil. Use of MA media  and  provide the necessary elements by used trace elements  for bacterial  growth and Poly-β-hydroxybutyrate  production in addition to using glucose as carbon source (1%). Gas chromatography(GC) state that obtain biopolymer was homopolymer poly-β-hydroxybutyrate (PHB) composition . Fourier Transform Infrared Spectroscopy (FTIR) FTIR show that, the functional group  of  extracted polymer, peak at 1743.71  cm־¹ be related  to the  (C=O) characters to the amorphous  phase of the extracted polymer.  In addition,  the thermal properties of the extracted polymer were studied using of  Differential Scanning Calorimetry Analysis (DSC) and  Thermo gravimetric Analysis (TGA) ,  the temperature melting (Tₘ) was (142-182°C), While the degradation occur in two reactions from (210.6-447.9°C). Molecular weight was 6.4* 10⁶ Dalton.