Isolation and characterization of virulence-related properties of pathogenic Vibrio Parahaemolyticus isolated from aquatic environments

Abstract

Vibrio parahaemolyticus is a pathogenic Gram-negative halophilic bacterium, a natural inhabitant of the marine environment and can be found in crabs, shrimps, fish, oysters, mussels and other seafoods. We areisolate and study the hemolytic activity of the pathogenic V. parahaemolyticus by different blood samples (human, rabbit and rat). Visualization of hemolysis of red blood cells in agar plates facilitates the categorization of some pathogenic bacteria such as vibrio sp. Haemolysins are one of the pathogenic determinants of several vibrio sp strains isolated from a variety of hosts and geographic origin.SDS – PAGE had been used to characterize and identify the virulent protein profiles on whole cell and extra cellular products (WC,ECP) of several species. The molecular weight was determined to be48.4kDaand 25.6kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Based on the results Hemolytic activity has been considered as important virulence factors contributing to the pathogenicity of the infection for the pathogens.